16S and 18S ribosomal RNA (rRNA) sequencing are common amplicon sequencing methods used to identify and compare bacteria or fungi present within a given sample. 16S and 18S sequencing are well-established methods for comparing sample phylogeny and taxonomy from complex microbiomes or environments that are difficult or impossible to study.
The TGC offers a varied of rRNA sequencing libraries from genomic DNA:
- Bacterial 16S sequencing libraries- V4 region (300bps single-end sequencing).
- Bacterial 16S sequencing libraries- V3-V4 (250bps paired-end sequencing).
- Fungi-specific 18S sequencing libraries.
The TGC also offers a flexible PCR amplicon sequencing workflow from user-provided PCR amplicons.
For user-prepared PCR amplicons, the PCR primers must contain the following over-hang sequences:
Forward overhang: 5’ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG‐[locus‐specific sequence]
Reverse overhang: 5’ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG‐[locus‐specific sequence]
For orders please read the samples delivery instructions, fill and send us the electronic sample sheet.
For additional information, please contact:
Liat Linde, tel 073-3785452 or 073-3785168
Rappaport building 073-3785221
Emerson building 073-3781387
