16S/18S amplicon-seq

16S and 18S ribosomal RNA (rRNA) sequencing are common amplicon sequencing methods used to identify and compare bacteria or fungi present within a given sample. 16S and 18S sequencing are well-established methods for comparing sample phylogeny and taxonomy from complex microbiomes or environments that are difficult or impossible to study.

The TGC offers a varied of rRNA sequencing libraries from genomic DNA:

  • Bacterial 16S sequencing libraries- V4 region (300bps single-end sequencing).
  • Bacterial 16S sequencing libraries- V3-V4 (250bps paired-end sequencing).
  • Fungi-specific 18S sequencing libraries.


The TGC also offers a flexible PCR amplicon sequencing workflow from user-provided PCR amplicons.

For user-prepared PCR amplicons, the PCR primers must contain the following over-hang sequences:

Forward overhang: 5’ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG‐[locus‐specific sequence]

Reverse overhang: 5’ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG‐[locus‐specific sequence]


For orders please read the samples delivery instructions, fill and send us the electronic sample sheet.

For additional information, please contact:

 Liat Linde, tel 073-3785452 or 077-8875168

Rappaport building 073-3785221

Emerson building 077-8871387