Illumina Sequencing Technology:
Explanations by the TGC:
- TGC brochure
- Sample Pooling: How to Choose Appropriate Indexed Sequencing Adapters
- How to Submit Samples to the TGC
- How to calculate sequence coverage
Standard Protocols at the TGC*:
- DNA Preparation–gDNA, ChiP, cDNA, amplicons:
- Exome Preparation
- RNA Preparation–purified mRNA, ribosomal-depleted RNA
- Single Cell or Low Input RNA
- CEL-Seq
- SMARTer Stranded Total RNA Sample Prep Kit – Low Input Mammalian
*If the protocol you want does not appear on this list: contact us! We’re always happy to introduce new protocols for the benefit of your research.
Reviews on Sequencing Technology/Methods:
- “An introduction to high-throughput sequencing experiments: design and bioinformatics analysis,” Methods in Molecular Biology. 2013;1038:1-26, Rachelly Normand and Itai Yanai