User-prepared libraries runs

The Technion Genomics Center offers sequencing services for user-prepared libraries on our two Illumina sequencers: Miseq and Nextseq 2000.

It is the responsibility of the user to make sure the libraries are compatible with Illumina sequencing, especially in terms of sequencing primers for custom library preparations*.

It is also the user’s responsibility to determine the percentage of the PhiX Control v3 library to be added to the run (other than the standard 2%), as it is important to compensate for low base diversity in libraries by spiking in PhiX Control v3 Library**.

The following flow cells are available for the Nextseq 2000 sequencer:

Flow cell Max read length
(single read)
Max read length (paired-end) Number of expected reads (millions)
P1 100 cycles 100 bps 2*50 bp 100 M
P1 300 cycles 300 bp 2*150 bp
P1 600 cycles - 2*300 bp
P2 100 cycles 100 bp 2*50 bp 400 M
P2 200 cycles 150 bp 2*100 bp
P2 300 cycles - 2*150 bp
P2 600 cycles - 2*300 bp 300 M
P3 100 cycles 100 bps 2*50 bp 1200 M
P3 200 cycles 150 bp 2*100 bp
P3 300 cycles - 2*150 bp
P4 50 cycles 50 bp 1800M
P4 100 cycles 100 bps 2*50 bp
P4 200 cycles 150 bp 2*100 bp
P4 300 cycles - 2*150 bp

The following flow cells are available for the Miseq sequencer:

Flow cell Max read length
(single read)
Max read length (paired-end) Number of expected reads (millions)
Nano 300 cycles 300 bp 2*150 bp 1 M
Nano 500 cycles - 2*250 bp
Micro 300 cycles 300 bp 2*150 bp 4 M
300 V2 300 bp 2*150 bp 12 M
500 V2 - 2*250 bp
150 V3 150 bp 2*75 bp 20 M
600 V3 300 bp 2*300 bp

 

For orders please read the sample delivery instructions 

Fill and send us the electronic sample sheet.

For convenient access to your sequencing data, we use the Illumina Basespace Cloud platform. Please refer to our detailed instructions on how to download your data here

* For more information please visit: Nextseq 2000- https://emea.support.illumina.com/bulletins/2021/03/sequencing-primer-compatibility-of-illumina-libraries-and-recomm.html; Miseq- https://emea.support.illumina.com/bulletins/2016/06/sequencing-primer-compatibility-with-illumina-library-prep-kits-recommended-for-the-miseq-sequencing-platform.html. For custom sequencing primers, please contact us.

** For more information please visit https://emea.support.illumina.com/bulletins/2017/02/how-much-phix-spike-in-is-recommended-when-sequencing-low-divers.html

 

For additional information, please contact:

 Liat Linde, tel 073-3785452 or 077-8875168

Rappaport building 073-3785221

Emerson building 077-8871387

Nextseq2000
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