The Chromium Single Cell 3’ mRNA counting allows you to perform deep profiling of heterogeneous cell populations with massive throughput digital gene expression on a cell-by-cell basis. Perform trace expression profiles from hundreds of thousands of single cells to ensure biologically relevant signals are not masked by bulk average measurements.
The optimal input cell concentration depends upon the desired cell recovery target, see below:
optimal input cell concentration | cell recovery target, |
700 - 1,200 cells/μl | 500 - 10,000 cells |
1,300 - 1,600 cells/μl | 10,000 - 20,000 cells |
Multiplet Rate (%) | # of Cells Loaded | # of Cells Recoveres |
~0.20% | ~725 | ~500 |
~0.40% | ~1,450 | ~1,000 |
~0.80% | ~2,900 | ~2,000 |
~1.20% | ~4,350 | ~3,000 |
~1.60% | ~5,800 | ~4,000 |
~2.00% | ~7,250 | ~5,000 |
~2.40% | ~8,700 | ~6,000 |
~2.80% | ~10,150 | ~7,000 |
~3.20% | ~11,600 | ~8,000 |
~3.60% | ~13,050 | ~9,000 |
~4.00% | ~14,500 | ~10,000 |
~4.40% | ~15,950 | ~11,000 |
~4.80% | ~17,400 | ~12,000 |
~5.20% | ~18,850 | ~13,000 |
~5.60% | ~20,300 | ~14,000 |
~6.00% | ~21,750 | ~15,000 |
~6.40% | ~23,200 | ~16,000 |
~6.80% | ~24,650 | ~17,000 |
~7.20% | ~26,100 | ~18,000 |
~7.60% | ~27,550 | ~19,000 |
~8.00% | ~29,000 | ~20,000 |
For details on sample delivery please read the sample delivery instructions and use the links below. Please fill out and send us the electronic sample sheet
- Cell prep
- Cell thawing protocols
- Nuclei prep
- Nuclei Isolation using 10x kit
- Cell counting
- Dead cell removal
- Cell fixation GEM-X fresh
- Tissue fixation dissociation GEM-X Flex
- Cell and nuclei fixation GEM-X Flex
- Cells from FFPE tissue sections
For additional information, please contact:
Liat Linde, tel 073-3785452 or 073-3785168
Rappaport building 073-3785221
Emerson building 073-3781387


